BIOL 41100 - Laboratory In Molecular Biology

Credit Hours: 3.00. This course is divided into two major sections. In the first section, students will study topics dealing with protein biology and biochemistry such as enzyme action and isolation, molecular evolution, and the detection and molecular basis of human disease. Techniques that will be used for these experiments include electrophoresis (both native and SDS denatured), chromatography (affinity and gel filtration), peptide mapping analysis, and the Western blot procedure. In the second section of the course, students will perform experiments that deal with DNA structure and function. These experiments stress the organization and complexity of the prokaryotic and eukaryotic genomes, gene function and regulation, and the structure of the eukaryotic chromosome. Techniques that will be used in this section include restriction nuclease mapping, isolation of organelles, gene cloning, and Southern blot hybridization procedures. Typically offered Fall.

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